Ribonucleates and the Gram stain

Ribonucleates and the Gram stain.

Salton (J. Gen. Microbiol. 30:223, 1963) summarized the evidence which demonstrates that the gram-positive cell is able to retain the crystal violet after iodine treatment because of the characteristic nature of its cell wall. This evidence is highly convincing, yet it does not explain the experiments of Henry and Stacy (Proc. Roy. Soc. London Ser. B 133:391, 1946) or of Bartholomew and Umbreit...

The Gram stain.

Mechanism of gram stain reversal.

This paper deals with an investigation of the effect of these reactions on bacteria. Not only the common staining behavior but also certain other similarities (Fischer et al., 1949; Fischer, 1951) between intact wool and gram negative bacteria, medium degraded wool and gram variable bacteria, as well as between highly degraded wool and gram positive bacteria suggested that reversal of gram stai...

Gram-Positive Diplococci in a Cerebrospinal Fluid Gram Stain

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Gram-Positive Rods on a Cerebrospinal Fluid Gram Stain

Cerebrospinal fluid (CSF) access device placement in the pediatric population presents challenges due to the development of infections following placement, access or revision, and/or shunt malfunctions. Here we report an unusual pediatric case of L. monocytogenes ventriculitis/VP shunt (VPS) infection and associated pseudocyst with an emphasis on the importance of VPS removal in clearing the in...